FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3511906
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG344220.9801515188618375No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA167920.47814491617941934No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC161150.4588676348398847No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC146310.41661137855056485No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG93730.266892109299053No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT76670.21831449930607483No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG67150.1912067122525489No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN56290.16028333332384181No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA46350.13197961448854267No Hit
GCGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG43370.1234941937511995No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC37670.10726369099856316No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA35890.10219521820914342No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC35390.10077148989750864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG69300.064.30474
TCTACGG70000.063.710675
TACGGCC70100.063.4240387
CTACGGC70750.062.8898286
CCTGGTT103650.061.2500770
ACGGCCA74150.060.1449368
GGGTCTA52700.059.6443632
GCCATAC74800.059.20782511
CGGCCAT75600.059.082129
GCGTCTA13750.058.3979642
CCATACC75800.058.20043612
CATACCA77200.057.23385613
GGCCATA78150.057.06487310
TACCACC79350.055.4685215
CGTCTAC16800.055.1358033
GCGCGGT81000.054.0421379
ATACCAC82250.053.8449314
CGCGGTG83550.052.432310
CGGTCTA9950.052.420972
ACCACCC83800.052.40018516