FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3601419
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG347830.9658137528568601No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA173100.4806438795374823No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC164260.4560979991497796No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC146240.40606216605177015No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG94710.262979675511236No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT77980.216525763872518No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG66660.18509370889641No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN57960.16093656417095595No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA47540.13200352416644662No Hit
GCGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG43800.12161872861780315No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA37930.10531959763637612No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC36940.10257068116761754No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC36690.10187651034217346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG71950.063.001384
TCTACGG72200.062.7832345
TACGGCC72350.062.2322657
CTACGGC73500.061.5819786
CCTGGTT107600.060.29592570
ACGGCCA76100.059.1656348
GGGTCTA57700.058.8172262
CGGCCAT76250.058.6891869
GCGTCTA12600.058.281042
GCCATAC77500.057.697511
CCATACC78100.057.38606312
CGTCTAC14650.057.1510473
CATACCA79350.056.56777213
GGCCATA80350.055.82259810
GCGCGGT79950.054.7712369
TACCACC82750.054.03092215
ATACCAC83700.053.62339414
CGGTCTA8850.053.508152
GCCGGTT12500.052.9808963
CGCGGTG83650.052.2665510