FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3568819
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG343080.9613264219900197No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA175800.4925999329189853No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC162930.45653758288105956No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC145840.40865059281515814No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG91680.2568917056314708No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT78410.21970853663354742No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG66110.1852433536136184No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN56300.15775526861967504No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA48110.1348065004137223No Hit
GCGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG44640.12508339593574233No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC38110.10678602641378003No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA36850.1032554466897873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG66200.064.284934
TCTACGG66400.063.9380345
TACGGCC67450.062.9935877
CTACGGC67800.062.6177836
CCTGGTT99750.060.8748270
ACGGCCA70100.060.759118
CGGCCAT70750.059.617119
GGGTCTA52950.059.047012
GCCATAC70750.058.98649611
GGCCATA73350.057.69104810
CCATACC72950.057.39578212
CATACCA73300.057.21536613
CGTCTAC14250.056.3568153
GCGTCTA12850.056.0869262
TACCACC75450.055.2665715
ATACCAC77500.054.11466214
GCGCGGT79150.054.0272189
CGGTTCA13200.053.561285
GCCGGTT12950.053.2686583
CTGAACG79450.053.04486522