FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3293976
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC158570.48139391422402594No Hit
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG140820.4275076685440331No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC117670.35722786079801433No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG80000.2428675861633479No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT55370.16809472807330714No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG51590.15661923462708896No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT48310.1466616635943917No Hit
TGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGG47490.1441722708362174No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG47050.14283649911231897No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT44740.1358236975618523No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA42500.12902340514927857No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG38600.11718361032381536No Hit
GTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCCTGCACTGGTGGGGTGAATTCTTTGCC38380.11651572446186614No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37500.11384418101406933No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG37220.11299414446249759No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA37050.11247805084190049No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC36690.11138514670416541No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN36300.11020116722161911No Hit
TGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCG34140.10364374239520871No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT33370.10130614187838648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA44400.037.29657432
GCGGCAC45950.035.90083733
CGTACGT1550.035.63722619
TCAGGAT22550.035.37033570
CGGCACC46950.035.15012434
CCGTCGC46150.034.2668345
TCGCCGG45750.034.25090448
CCCGTCG47550.033.46074744
TGGCCGT13650.032.91440270
CCCCCGA51000.032.01891319
GGCACCC52450.031.47864335
GCACCCC52500.031.2638336
ACCCGCG53200.031.25913425
CACCCCC53750.030.54750437
CCCCGAC54300.030.26973320
CGATCTA1850.030.15815447
GTACGTA1950.030.10341820
AGGACCG5350.029.25071570
GATAGGC42300.029.16572240
CTGATAG43450.028.68956238
Produced by FastQC (version 0.11.5)