FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3293976
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA129240.3923525854468885No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG99010.3005789963254134No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC95070.28861776770686853No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC79370.2409550039223115No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT45720.1387988254923533No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN36170.10980650739410365No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA34460.1046152127398621No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC34050.10337051636077495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCC21850.058.8969577
TCTACGG22100.058.6981475
GTCTACG22300.058.0170024
CTACGGC22400.057.7579966
CGGCCAT25200.051.478139
GCGCGGT47050.051.4769679
GCCATAC25250.051.2356611
ACGGCCA25500.050.7372068
GCGTCTA4300.049.743042
CCATACC26100.049.4371412
CATACCA26300.048.9300113
CCGAACT8650.048.8918567
TGGCGCG49950.048.41629815
GGGTCTA21550.047.5464672
CGCGGTG51400.047.38894310
TGAACGC27300.047.2015623
GAACGCG27200.047.1332724
CGGTTCA9450.046.7312135
ATCTCGT27250.046.70807335
GGCCATA27900.046.37271510
Produced by FastQC (version 0.11.5)