FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3275467
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA129900.39658467021649124No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC99480.30371241719119746No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG96440.29443129788820954No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC77640.23703490219867887No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT45200.13799558963653122No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35940.10972481175966664No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA33800.10319139225032645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG20450.060.739484
TCTACGG20800.059.7174225
TACGGCC21000.059.310277
CTACGGC21950.056.274346
GCGCGGT43150.052.6922349
ACGGCCA24300.051.2557878
CGGCCAT24350.051.008859
GGGTCTA19450.050.392612
GCCATAC24900.050.1592711
CATACCA25150.049.6599113
CCATACC25800.048.27506312
TGGCGCG47800.047.99865715
GAACGCG25950.047.80535524
CCCGATC26000.047.68049231
CCTGGTT38000.047.0510270
GGCCATA26800.046.98941810
ATCTCGT26700.046.88064635
TGAACGC26650.046.66791523
CTGAACG26950.046.1399522
CGCGGTG49650.046.07191510
Produced by FastQC (version 0.11.5)