FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3271642
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC154460.4721176705764262No Hit
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG140410.4291728740491777No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC118200.361286473275499No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG81590.2493854767728254No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT56240.17190144887490746No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG49990.15279789170086458No Hit
TGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGG48370.14784624968135265No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT47910.14644022787334313No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG46930.14344479010845318No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT45210.13818749117415657No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA42080.12862042974139593No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39210.11984807628707542No Hit
GTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCCTGCACTGGTGGGGTGAATTCTTTGCC38390.11734168958584099No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG38340.11718886112844865No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG38010.11618019330965917No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN36260.11083119730092718No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA35850.10957800395030998No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC35830.10951687256735303No Hit
TGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCG35020.10704105155759708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT1600.038.83545319
GTTCCGT2600.038.802991
CGCGGCA41450.036.1880232
TGGCCGT13200.035.48604670
CGGCACC43750.034.22636834
GCGGCAC43900.034.1773433
CCGTCGC43100.033.06516645
TCGCCGG42850.032.90133348
AGGACCG6000.032.7387470
GTCCCGT1600.032.6145821
CGCCCTA8950.032.2841361
CCCGTCG44000.032.21103744
CCCCCGA45900.032.1889619
TTATCGG2050.031.9715127
CGATCTA2100.031.55649847
GCACCCC48650.031.02815236
GATAGGC44700.030.55932440
GACGTAT2300.030.28064544
ACCCGCG49850.030.22986425
TCAGGAT22500.029.9965270
Produced by FastQC (version 0.11.5)