FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3318303
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA188760.5688449788943325No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC164250.4949819229889495No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG146160.44046610571728984No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC138110.41620671771082984No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT85200.2567577463540852No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA44300.13350197374983538No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC39290.11840389500295784No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA39290.11840389500295784No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG38590.11629438300239611No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC37570.11322052265872043No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC33210.10008127648379306No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG28350.061.3941575
GTCTACG28350.061.2723434
TACGGCC28850.060.2104387
CTACGGC29750.058.5050166
GCGTCTA6750.055.765522
GCGCGGT77400.055.0138059
GCCATAC32200.053.93898411
ACGGCCA32550.053.578438
CGGCCAT32650.053.3085569
CCGAACT13600.053.25675667
GCCGGTT13750.052.993523
GGGTCTA24150.052.9087642
CGCGGTG80950.052.5585610
CCATACC33450.052.0328312
TGGCGCG81850.051.8954715
GGCCATA33800.051.5969810
CGAACTT14350.051.34913368
CCTGGTT50350.050.4346570
CATACCA35000.049.6298513
CGTCTAC8600.049.391213