FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3306194
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA189070.5718660187514707No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC167360.5062013904810183No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG146580.4433496642967714No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC144170.43606031587983035No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT85900.2598153647366126No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA43680.13211565927468263No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG38550.11659932841206536No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC38490.11641785085811662No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA38430.11623637330416788No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC38240.1156616943833302No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC33510.1013552138803712No Hit
GGCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC33330.10081078121852499No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG30400.061.4427154
TCTACGG30450.061.228445
TACGGCC30900.060.0006527
CTACGGC31750.058.3952146
GCGCGGT78000.055.018679
GCCATAC34350.054.6771311
ACGGCCA34450.054.2177668
CCATACC35250.053.57414212
CGGCCAT34950.053.4421169
CGCGGTG81300.052.91283810
GCGTCTA5750.052.252052
TGGCGCG82800.051.61991515
CATACCA36350.051.38304513
GCCGGTT12300.051.3683
CCTGGTT53200.051.2853970
GGGTCTA27000.050.7783552
GGCCATA37050.050.69256210
CGGTTCA13000.049.929965
CCGGTTC12900.049.7817274
CCGAACT13200.049.50680567
Produced by FastQC (version 0.11.5)