FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3311244
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA183800.5550783934980328No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC165180.4988457510228784No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG145210.4385360909676243No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC140080.42304342416324503No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT83400.2518690860594991No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA43840.1323973708974633No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC39330.11877711216690767No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG39020.11784090812999586No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA38130.11515309654015228No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC37200.11234448442941686No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC33750.10192543950249514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG28500.061.299324
TACGGCC28450.061.0402077
TCTACGG29050.060.49535
CTACGGC30100.058.2676476
GCGCGGT75600.054.4358189
ACGGCCA32050.054.18398
GCCGGTT12900.054.065253
GCCATAC32550.053.24551811
GGGTCTA24700.052.9783972
CGGCCAT32900.052.6790739
CGCGGTG78850.052.19211610
CCATACC33650.051.60754812
CGGTTCA13600.051.5355765
TGGCGCG80150.051.17327515
CATACCA34050.051.1026913
CCGGTTC13700.050.9073874
CCGAACT14100.050.85035767
GGCCATA35000.049.912910
CCTGGTT53500.047.8251870
GCCGAAC15350.046.98285366
Produced by FastQC (version 0.11.5)