FastQCFastQC Report
Wed 8 May 2024
EGAF00005318892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3310996
Total Bases248.5 Mbp
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC185950.5616134842808629No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCAC167460.505769260971623No Hit
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG115460.34871682116197056No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG100990.3050139595457077No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT71790.21682297411413365No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT71580.21618872387644078No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG66150.19978882487324054No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT66070.19954720573507187No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA62120.18761726078799248No Hit
TGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGT60320.18218083017919684No Hit
GTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCCT52060.15723365416327897No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG50890.1536999742675618No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT50210.15164621159312788No Hit
TGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCG46470.1403505168837413No Hit
GCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGA41110.12416203462643868No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG40130.12120220018387215No Hit
GTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGG39710.11993369970848651No Hit
GCACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACC39100.1180913537799502No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG37990.11473888823785953No Hit
GGGGCTGGGGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCA36720.11090318441943148No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGT35670.10773193323096737No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG35050.10585938491015996No Hit
GGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTG34890.10537614663382257No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)