FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3310996
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA178530.5392033092157164No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC158880.47985560840303043No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG143680.4339479721509781No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC137140.41419560760568724No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT81260.24542463959485303No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA42050.12700105949992088No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG37920.11452747149196194No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC37740.11398382843108236No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC36340.1097554935131302No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA35500.10721849256235888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG27750.062.064764
TCTACGG27800.061.837395
TACGGCC28400.060.530057
CTACGGC29250.058.653936
ACGGCCA30850.056.170548
CGGCCAT31600.054.9474459
GCGCGGT78200.054.4280669
GCCATAC31650.054.20542511
CCGAACT12700.053.72115367
CGGTTCA13100.052.9652945
CGCGGTG81200.052.50141510
GCCGGTT13100.052.4311373
TGGCGCG82700.051.6696815
GGCCATA33850.051.49828310
GGGTCTA25800.051.2505152
CCATACC33500.051.2073412
CATACCA33750.050.72575413
CCGGTTC14100.048.95744
CGAACTT14500.048.8632868
TACCACC35050.048.35196715