FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3157008
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC139650.4423492116586337No Hit
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG116690.36962212322553506No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC96740.30642937870287307No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG89000.2819124943617501No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN55710.17646455124598986No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT51340.1626223310172163No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG44210.14003765590711204No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG44190.13997430478478357No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT43330.1372502065246588No Hit
TGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGG42110.1333857880626213No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT36610.11596422942228843No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG35890.11368358901846305No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA34410.1089956059661553No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG33460.10598642765555234No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32090.10164687577605125No Hit
GTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCCTGCACTGGTGGGGTGAATTCTTTGCC31730.10050655557413855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT14650.042.751770
CGCGGCA34150.036.69425232
GCGGCAC36550.034.015333
CGGCACC36700.033.69830334
CCGTCGC35800.031.8238145
ATGGCCG15650.031.49026169
ACCCGCG40050.031.457125
CCCGTCG36650.031.35231244
TCGCCGG36600.030.99553948
CCCGTTA902.0294829E-730.6103763
CCCCCGA41300.030.45464119
CCCCGAC42500.029.60414520
GATAGGC43200.029.510440
GCACCCC42450.029.48606336
AGGACCG6550.029.2392470
GCATTAG43600.029.0635112
CCCCCGT39700.028.99645442
ATAGGCA44300.028.87407141
GGCACCC43350.028.8628335
TCAGGAT23250.028.71920470
Produced by FastQC (version 0.11.5)