FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3188987
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC140370.44017112644234674No Hit
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG123330.3867372303493241No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC99620.3123876014546312No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG88830.2785524055130987No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN58530.1835379071786746No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT52790.16553846095954608No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG45050.14126743069194073No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG42780.1341491827969195No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT42420.13302029766819368No Hit
TGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGG42400.13295758182770892No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG37540.1177176325899102No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT35020.10981543668882938No Hit
GTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCCTGCACTGGTGGGGTGAATTCTTTGCC34340.10768309811234728No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA33230.10420236896544262No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32560.10210138830920289No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT15000.037.84428470
CGCGGCA35050.036.4598332
GCGGCAC35800.035.7112433
CGGCACC37000.034.47192834
CCGTCGC35850.033.91779345
TCGCCGG36600.033.46953248
CCCGTCG36850.033.07561544
CCCCCGT38500.031.89701742
ACCCGCG41250.031.1391925
CCCCCGA41850.030.8082719
GTGCCGT4800.030.1908261
GCACCCC42750.029.94388636
GTTCCGT3150.029.5746861
GGCACCC43150.029.49431235
CCCCGAC43600.029.3432620
TCAGGAT22850.029.1341570
ATGGCCG15950.028.76440869
AGTCCGC46600.028.0153647
CACCCCC46400.027.8228137
GATAGGC41850.027.57225440