FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3683530
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG184140.4999009102681395No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC110920.30112419336886087No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC92610.2514164402081699No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG81430.22106511959994898No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG51450.13967580011564992No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC48890.13272594494954568No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA47010.12762214506193786No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG46570.12642763870526372No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG45090.12240975368735968No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT44920.12194823986773556No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG37220.10104437862593761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA57200.039.93269732
GCGGCAC60350.037.858733
CGGCACC61050.037.6635734
GCACCCC65750.035.2575936
CCCCCGA65750.034.92405319
TCGCCGG58950.034.835548
CCGTCGC58400.034.5197445
CCCGTCG59300.034.09553544
GGCACCC68550.033.80547335
CACCCCC69050.033.3833937
ATGGCCG12300.032.56439269
TGGCCGT15500.032.52699370
ACCCGCG69700.032.22909525
TAGTCCG4200.031.2746126
CCCCGAC74200.031.09174720
GTTCCGT3650.030.425311
ACGATCG703.8028644E-529.6285786
GTACGGG10000.029.3816745
TACGGGT10000.029.0360076
CGCGTTT605.349918E-428.8180923
Produced by FastQC (version 0.11.5)