FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3683530
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA94230.25581439543047024No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC93590.2540769316389442No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG92360.25073774341460503No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC81820.2221238865979102No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT45030.12224686645690411No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC38290.10394920090239525No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCC20400.061.493797
GTCTACG20800.060.311224
TCTACGG21000.060.0659565
CTACGGC21950.057.15146
GCCATAC23750.052.6651211
ACGGCCA24450.051.731738
GCGCGGT45800.050.8568239
ATCTCGT24750.050.1703335
CATACCA25250.049.54255313
CCATACC25700.049.48184212
CCCGATC25200.049.08190531
CGGCCAT25750.048.7174079
GAACGCG25750.048.63408724
TGGCGCG49250.047.43202215
CCGAACT9300.047.3089667
GGGTCTA20400.047.2612532
TCTCGGA26600.047.1359546
CGCGGTG49800.047.04951510
GCCCGAT26400.046.8413430
TGAACGC26900.046.80832323
Produced by FastQC (version 0.11.5)