FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3670086
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA93590.2550076483221374No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG92930.25320932534006013No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC91810.2501576257341109No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC82730.22541706107159343No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT45730.12460198480362586No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC38270.10427548564257078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG20250.060.1021044
TACGGCC20450.059.3436167
TCTACGG20650.058.9379045
CTACGGC21650.056.21566
GCGCGGT45400.050.2623029
GCCATAC24150.050.10778411
ACGGCCA24400.050.019488
CGGCCAT25050.048.5835469
TGAACGC25200.048.0632223
GAACGCG25400.047.55501624
CCGAACT7350.047.2863567
ATCTCGT25650.046.93441835
CCATACC25850.046.6787512
CCCGATC25900.046.29738231
TGGCGCG50100.045.9611415
CGCGGTG49850.045.7061410
CATACCA26700.045.3222113
GCGTCTA5250.044.7954862
CTGAACG27000.044.72290422
CCTGGTT38150.044.2350770
Produced by FastQC (version 0.11.5)