FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3682685
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG93420.2536736104228301No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC93260.2532391448087469No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA91220.24769970822918602No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC85520.23222187072747194No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT45850.12450155253571782No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC38600.10481482939757271No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG18750.059.352424
TACGGCC18750.059.3491947
TCTACGG18800.059.0107355
CTACGGC19450.057.2109076
GCGCGGT49200.050.574049
GCCATAC22050.050.46700711
ACGGCCA22800.049.261638
CCGAACT8050.048.49558667
CGGCCAT23050.048.4274759
TGGCGCG52450.047.5035915
ATCTCGT23900.046.73070535
CCATACC23750.046.56360612
GAACGCG23950.046.52530324
CATACCA23800.046.3199513
CGCGGTG53900.046.22817610
TGAACGC24350.045.89689623
GGGTCTA19600.045.846592
CCCGATC24250.045.58363731
GCCCGAT25000.044.3432630
GCCGGTT9000.044.161633
Produced by FastQC (version 0.11.5)