FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3681796
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG183050.4971758348371284No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC112830.30645369814079865No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC92900.2523225078195533No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG83280.22619395534136058No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG52640.14297370087859293No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC49640.13482550364007131No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG46530.12637853916947053No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA46410.1260526112799297No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT45150.12263036843975061No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG44970.1221414766054393No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG38100.10348210492922476No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT12800.037.30039270
CGCGGCA58250.037.0275532
GCGGCAC60100.035.83593433
CGGCACC60600.035.6620134
GCACCCC64050.033.821136
CCCCCGA65700.032.95190419
GTGCCGT6150.032.8847121
TCGCCGG57350.032.77825548
CCCGTCG57700.032.33867344
CCGTCGC57400.032.2783545
GGCACCC67800.032.18934235
CACCCCC67300.032.09343337
ACCCGCG68900.030.69820825
GATAGGC34050.030.32012640
CCCCGAC72650.029.70801520
ACGATCG703.8020757E-529.6294786
ATGGCCG13050.029.47147469
CGCTTCC8450.028.8856721
CATTAGC36450.028.26036313
ATAGGCA36900.027.98368541