FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3681796
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG88960.2416212087796282No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC88710.24094219234308475No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA87950.2388779823759926No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC79370.21557413827382071No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT45570.12377111605314363No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC37040.10060307523828045No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG20000.059.1016045
TACGGCC20400.058.111387
GTCTACG20550.057.6872184
CTACGGC22100.053.4856156
GCGCGGT46450.051.1917539
GCCATAC24000.048.96265411
ACGGCCA24550.048.8512048
CCATACC24750.047.89396312
ATCTCGT24450.047.24800535
GAACGCG24800.047.15577724
TGAACGC24900.046.95808823
CGCGGTG50700.046.90052810
CGGTTCA8550.046.8915985
CCGAACT8700.046.87701467
CCCGATC25150.046.57179331
GCCGGTT8650.046.34953
CGGCCAT25700.046.261819
GGGTCTA20150.045.8003652
TGGCGCG51550.045.7876115
GCCCGAT26150.045.1773130
Produced by FastQC (version 0.11.5)