FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3290250
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA125810.38237216017019987No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG123190.374409239419497No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC119990.36468353468581416No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC104950.31897272243750474No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT50170.15248081452777146No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA48300.14679735582402553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG26300.060.5254444
TCTACGG26350.060.4105955
TACGGCC26500.060.0668227
CTACGGC26900.059.047076
ACGGCCA29200.054.866618
CGGCCAT29300.054.561519
GCCATAC29700.053.2453911
GCGCGGT57250.053.07389
CCATACC30700.051.39776212
GGGTCTA24250.050.988992
CCTGGTT44300.050.78968470
CATACCA31700.049.8860613
CGCGGTG61050.049.7702710
GGCCATA31950.049.71185310
TGAACGC32150.049.23948323
TGGCGCG61750.049.2060715
GAACGCG32700.048.42088724
CCGAACT8300.048.37909767
CTGAACG33300.047.52889322
ATCTCGT33250.047.020335
Produced by FastQC (version 0.11.5)