FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3296236
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG127600.38710820463097906No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA124100.37649003287385974No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC119540.36265607195601285No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC104500.3170282710339915No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT51430.15602644956247064No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA47470.14401274666012992No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG33820.10260187680736452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG25650.060.0358354
TACGGCC25800.059.8206147
TCTACGG26200.058.6437575
CTACGGC27150.056.7163546
ACGGCCA28700.053.6557168
GCGCGGT53150.053.268899
CGGCCAT29400.052.1433229
GCCATAC29800.051.32832711
GGGTCTA23650.051.0998652
CCGAACT8150.051.0423467
GCGTCTA4900.050.0316852
CCTGGTT43700.049.79633370
CGCGGTG57100.049.6443810
TGGCGCG57900.049.01659415
CCATACC31300.048.86850412
CATACCA31950.048.3065813
GGCCATA32100.047.97256510
TGAACGC32650.046.8963223
ATCTCGT32750.046.2520635
GAACGCG33250.045.74517424