FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3299237
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC175220.5310924919913301No Hit
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG142650.43237269708117365No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC134250.4069122648660888No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG87090.2639701240013979No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT64170.19449951610023772No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG55330.167705442197696No Hit
TGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGG53150.16109785383711447No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG53080.1608856835686554No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT45790.1387896656105639No Hit
GTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCCTGCACTGGTGGGGTGAATTCTTTGCC43610.13218207724998235No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA42820.12978758422023032No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT40220.12190697424889453No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG39940.12105829317505835No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG37630.11405667431591002No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG36600.11093474036572698No Hit
GCACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACC36090.10938892840981111No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT15150.041.15106670
CGCGGCA45050.039.5204632
GCGGCAC46700.038.0592233
AGGACCG5800.037.88561270
CGGCACC47250.037.5571734
GCACCCC51850.034.45144336
TCAGGAT24500.034.2067470
CCCGTCG46300.033.8121844
TCGCCGG46200.033.72235548
CCGTCGC46550.033.34592445
CCCCCGA53900.033.31968719
ATGGCCG14650.033.2327569
GGCACCC54150.032.9095335
ACCCGCG53100.032.87648825
CACCCCC55000.032.43057337
TCGCCGT3750.031.5840051
CCCCGAC57100.031.33474220
CCCCCGT50700.030.77905842
GGTAGAC11250.029.52532870
AGTCCGC61300.029.5079947