FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3299237
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG121220.3674182848943559No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA117450.355991400435919No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC114450.34689838893053154No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC97590.295795664270254No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT49680.1505802705292163No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA44700.1354858714302731No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG25000.062.280645
GTCTACG25150.061.7709774
TACGGCC25200.061.645617
CTACGGC25950.059.734546
GCCATAC28850.054.68403611
CGGCCAT28800.053.9399079
GCGCGGT55550.053.6311269
CCATACC29300.053.25264712
ACGGCCA29800.052.8249058
GGGTCTA23100.051.5753672
CCTGGTT43250.051.11076770
CATACCA31100.050.50349413
CCGAACT8550.050.3424767
CGCGGTG59250.050.1654810
GGCCATA31450.049.9437210
GAACGCG31000.049.2763724
TGAACGC31000.048.93448323
TGGCGCG60550.048.74339315
GCCGGTT9000.048.715213
CTGAACG31500.048.3690622
Produced by FastQC (version 0.11.5)