FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3060112
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG130320.4258667656608647No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG55030.1798300192934115No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA35010.11440757723900302No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG34660.11326382825203782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA37250.033.36791632
TCGCCGG37350.031.52948648
CCCGTCG37350.031.40573344
CCGTCGC37750.031.07806445
GCGGCAC40200.031.00716233
CGGCACC42200.029.54101834
TACGGTC952.9972762E-729.2776971
CGCGACT755.9797694E-527.7250922
CGTACGT1404.456524E-1027.22777619
ACCCGCG46150.026.9016325
CCCCCGA47600.026.64536319
GCACCCC47400.026.53044536
CACCCCC47250.026.47544137
GTGCCGT5150.024.9784221
CCCCGAC50650.024.97570620
TACCGCG1001.4833831E-524.31713536
GGCACCC52250.023.93052535
GTCCGTT2053.6379788E-1223.6677652
CGTCGCC49900.023.58653846
AACGGCG3700.023.54819749
Produced by FastQC (version 0.11.5)