FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3049242
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG131450.4310907432076562No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG54850.1798807703685047No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG34780.1140611338817975No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA34650.11363479841875457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA38800.035.34107632
TCGCCGG38550.034.1426948
CCCGTCG39350.033.41604244
GCGGCAC40950.033.4084833
CCGTCGC39900.032.96302445
GTTCCGT4550.031.5047071
AGGACCG4800.031.44964470
CGGCACC43800.031.1584434
AATCGCG3400.029.72206350
CCCCCGA46150.029.65744219
ACCCGCG47100.029.15291825
GCACCCC47750.029.02731136
CACCCCC48600.028.451937
AACGGCG4100.028.03566449
CGCCCTA6700.027.18327361
CCCCGAC51900.026.23988720
GTGCCGT4750.025.7618711
ATCGCGC3700.025.43948751
AGTCCGC55500.025.2779627
CGTCGCC52550.025.16546246
Produced by FastQC (version 0.11.5)