FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3049242
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC85060.2789545729725617No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC64260.21074089888569028No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT38990.12786784387726524No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC36670.12025939561372957No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT40250.050.0669759
TGGCGCG41900.047.35162715
CGCGATA306.013045E-446.4567871
CCGAACT7300.043.98362767
CGCGGTG47500.042.42517510
CGGCATT4700.041.5145761
CGAACTT8000.041.39413568
CGGTAAG2500.040.4174081
CGGTTCA8350.039.3973545
CTACTCG51750.038.9169937
TACTCGG52400.038.6406738
CGTGCCT51700.038.5218420
CGGGTAG4900.038.397961
GCCGGTT8500.038.2947163
GTAGTCC53850.037.59787827
ACTCGGG55450.037.0218139
TGTAGTC54650.036.85294726
GCGTGCC54650.036.50521519
TAGTCCC55350.036.3958428
CGGCATA2400.036.2943651
Produced by FastQC (version 0.11.5)