FastQCFastQC Report
Wed 8 May 2024
EGAF00005318935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3360169
Total Bases247.8 Mbp
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT371701.106194361057435No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG287160.8545998728040167No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN157340.46825025765073125No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTG120060.35730345705826105No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT82110.24436270913754635No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA81700.24314253241429226No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG79510.23662500308764234No Hit
GGCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT71220.21195362495160217No Hit
GGGGACTCTTCTGGTCCGCACAGACTCAGAGAGAACCCACCATGGTGCTG58110.17293773021535525No Hit
GCGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT53650.1596645882989814No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG53620.15957530707532866No Hit
GCGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG35870.10675058308079147No Hit
GGGTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGG35090.10442927126582027No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)