FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3339270
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG159050.4763017066604378No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN156020.4672278671685728No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG104970.31435014239639203No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC84690.2536183057973749No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC67270.2014512153853986No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT63570.19037094933922685No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA61300.1835730563865755No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT59700.17878158998823096No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG45310.13568833906811967No Hit
TGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCG44710.13389153916874047No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG43230.12945943275027177No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG43000.12877065945550975No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT42900.1284711928056132No Hit
GTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGG36560.10948500720217294No Hit
GGGGCTGGGGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCA36090.10807751394765922No Hit
GCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCAGCTT35970.10771815396778339No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCG35010.10484327412877664No Hit
GGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTG34140.10223791427467681No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT34140.10223791427467681No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT28400.049.60865470
CGCGGCA45050.036.21259332
GCGGCAC48100.034.1089633
TCGCCGG47100.033.52227448
ATGGCCG33450.033.39930769
CGGCACC49700.033.17952334
CCCGTCG48100.032.84224344
CCGTCGC47750.032.7586745
GTTCCGT3050.032.5318261
ACGATCG652.6095682E-531.308366
CCCCCGA53500.030.30193119
GCACCCC54900.030.1768236
ACCCGCG54300.029.92830525
TTATCGG2500.029.8473057
GTTATCG2300.029.4933826
CACCCCC56900.029.20783837
CCCCGAC55900.029.02240220
CCCCCGT53750.028.91242842
GGCACCC58000.028.5267135
AGTCCGC58600.028.3030787
Produced by FastQC (version 0.11.5)