FastQCFastQC Report
Wed 8 May 2024
EGAF00005318937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3339270
Total Bases246.3 Mbp
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT377141.1294085234197895No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG286260.857253231993819No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN155120.46453266731950393No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTG117910.35310112689300355No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT83030.2486471594090924No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA82060.24574233290509603No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG78710.23571020013356211No Hit
GGCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT73010.21864060108945965No Hit
GGGGACTCTTCTGGTCCGCACAGACTCAGAGAGAACCCACCATGGTGCTG59510.17821260335342753No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG54040.16183177760408712No Hit
GCGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT50410.15096113821284293No Hit
GCGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG35390.10598124739838348No Hit
GGGTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGG33850.10136946098997686No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)