FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3350381
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG163170.48701923751358434No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN155560.4643054028780607No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG106570.3180832269523974No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC85710.2558216513286101No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT65120.19436595420043273No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC65100.1943062594970542No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA61340.1830836552618941No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT58810.17553227528451243No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG46220.13795445950773957No Hit
TGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCG43890.131000026564143No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT43310.12926888016616617No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG43030.128433154318867No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG42890.12801529139521745No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCG36530.10903237572085087No Hit
GGGGCTGGGGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCA36250.10819664987355171No Hit
GTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGG35770.10676397699246741No Hit
GCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCAGCTT35330.10545069351814017No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34260.10225702688738982No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT34170.10198840072218653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT30700.046.37607270
GTTCCGT4000.038.346451
CGCGGCA48000.036.0191632
GCGGCAC50200.034.4773133
GACGTAT3850.033.58321444
CGGCACC52100.033.3191134
ATGGCCG34150.033.14594769
TCGCCGG49700.033.0135648
CCGTCGC50100.032.6797445
GGACGTA4000.032.28699543
CCCGTCG50900.031.9925944
AGGACCG6650.031.4848670
ACGTATC4150.031.19062845
CCCCCGA57000.030.33116319
GCACCCC58400.030.2680536
ACCCGCG57700.030.09972625
CGTTCGT2500.029.9954431
CGGATAG4750.029.82425935
CGTATCA4500.029.57819246
CCCCGAC59450.029.27402920
Produced by FastQC (version 0.11.5)