FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3350381
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG280600.8375166884005133No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC176790.5276713305143504No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN154450.46099234684055335No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC151160.4511725681347883No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA109080.32557491222640056No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT76980.2297649133038899No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG75580.22558628406739414No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG54480.16260837200306472No Hit
GGGGACTCTTCTGGTCCGCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA53150.15863867422839373No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC40390.12055345347290353No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC35240.10518206735293688No Hit
GCGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG34700.10357031036171706No Hit
GGCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC34630.10336137889989229No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG57150.063.141974
TCTACGG58000.062.2166145
TACGGCC58700.061.5903437
CTACGGC59700.060.4990966
ACGGCCA61800.058.6107258
CGGCCAT62100.058.163589
GGGTCTA44500.057.827762
GCCATAC62750.057.29059611
CCATACC64450.055.8847912
CATACCA64800.055.8448713
GGCCATA66050.055.09638610
GCGTCTA11800.054.951372
CCTGGTT97150.054.1569470
GCGCGGT86450.053.2078139
CGTCTAC14100.052.9685063
TACCACC68850.052.45890815
CCGAACT11850.051.85912767
CGGTCTA7200.051.3950352
ATACCAC70450.051.2193314
GCCGGTT11900.050.20883
Produced by FastQC (version 0.11.5)