FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3349014
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG272170.8126869580121194No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC168150.502088077266921No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN157700.47088486342547387No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC147450.4402788402795569No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA106280.31734713560468847No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG75550.2255887852364905No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT74710.22308058431526412No Hit
GGGGACTCTTCTGGTCCGCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA51580.15401548037720952No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG51460.15365716595989148No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC38060.11364538935937563No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC34330.1025077828877395No Hit
GCGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG34300.10241820428340997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG59800.063.850975
GTCTACG59850.063.848594
TACGGCC61300.062.3439187
CTACGGC61750.061.779676
ACGGCCA64550.059.2049948
GGGTCTA46650.058.6495672
GCCATAC64850.058.6170911
CGGCCAT66050.057.911839
CCTGGTT94700.057.1104570
CCATACC66700.056.93782812
GGCCATA68000.056.20121410
GCGTCTA11000.056.164272
CATACCA67500.056.16245313
GCGCGGT85400.054.3681379
CCGAACT12200.054.27324367
TACCACC70000.054.10654415
CGTCTAC13700.053.7538723
CGGTCTA8300.053.1675532
GCCGGTT11700.052.7905433
ATACCAC72400.052.26608314