FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4388827
Sequences flagged as poor quality0
Sequence length35-76
%GC64

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA350150.7978213768735929No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC219590.5003387009786442No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC183130.41726411180026013No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN158230.3605291345500745No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT105110.2394945164163454No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA74900.1706606343790721No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC73980.16856440228790062No Hit
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA58360.13297402700083644No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG56580.12891827360704808No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA55220.12581949573314236No Hit
GGGGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGGCCGC54720.12468023916185349No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC50460.11497377317447237No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT48580.11069016846642624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGACT61550.054.7648741
CCGAACT17150.054.0987267
GCGCGGT109350.053.836899
CGGTTCA16800.053.5694855
GCCGGTT17100.052.629073
CGCGGTG114100.051.71564510
ACTCTTC79050.051.5562025
CGGGACT17050.050.934821
TCTACGG4250.050.725075
GGACTCT82150.049.9850043
TGGCGCG119000.049.7749915
TCCTTAG18200.049.07328416
CCGGTTC18350.049.0445484
TCTGGTC81050.048.9863710
GGGACTC81850.048.830952
TTCTGGT81750.048.7343949
TCTTCTG83550.048.5447467
CTTAGGC18350.048.353918
CCTTAGG18500.048.12003317
CATATTG19450.047.7930757
Produced by FastQC (version 0.11.5)