FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4330761
Sequences flagged as poor quality0
Sequence length35-76
%GC64

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA345710.7982661707722962No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC216840.5006972215737603No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC177560.4099972268153334No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN155120.358181853027678No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT102110.23577842323785592No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA73210.169046502450724No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC73190.16900032119066372No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA57030.13168586306194224No Hit
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA56620.1307391472307061No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG55760.12875335304811325No Hit
GGGGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGGCCGC53260.1229806955405759No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC50850.11741585370330988No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT48060.11097356792489818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTCA16850.056.0352975
CCGAACT16900.055.7901167
GCCGGTT16950.055.7027663
GGGGACT62800.055.5032581
GCGCGGT104900.054.297049
ACTCTTC81200.052.4524575
CGCGGTG109800.052.18550510
GGACTCT82800.051.3957823
CGGGACT16800.051.3069881
CCGGTTC18650.050.9921574
CCTTAGG18250.050.8259617
CTTAGGC18550.050.58627318
TGGCGCG113400.050.496915
TCTTCTG85200.050.1510937
TCTGGTC83350.050.1126610
GGGACTC81550.049.7925262
TTCTGGT84500.049.7545249
TCCTTAG18900.049.41094216
TTAGGCA19250.049.30853319
GCCGAAC19150.049.21951766
Produced by FastQC (version 0.11.5)