FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3308850
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN147610.4461066533689953No Hit
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG146760.4435377850310531No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG54710.1653444550221376No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG41280.12475633528265108No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA34630.10465871828580926No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT851.8189894E-1244.3207419
CGTACTA1004.2018655E-1034.185472
CGCGGCA45850.033.86520832
GCGGCAC47400.032.7109633
TCGCCGG45450.032.1780248
CGGATAG3900.032.00316235
CCCGTCG46400.031.62335244
CCGTCGC46800.031.37934945
CGGCACC49550.031.17778634
CCCCCGA54100.029.37338319
TGGCCGT15750.029.30249870
ACCCGCG54100.029.10285225
GGACGTA4350.028.89127243
GCACCCC54200.028.68413236
GTGCCGT4750.028.1569581
GACGTAT4150.027.78065344
CACCCCC56050.027.69892937
AACGACG500.00790652127.47765524
TAGTCCG3150.027.1309156
CCCCGAC59400.026.6546420
Produced by FastQC (version 0.11.5)