FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3308850
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN146110.4415733563020384No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC129770.39219064025265576No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC106490.3218338697734862No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG99770.3015246989135198No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT51670.1561569729664385No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC35800.10819468999803557No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA34660.10474938422714841No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG22450.059.6663975
GTCTACG22750.058.879594
TACGGCC23200.056.8529247
CTACGGC23850.055.877426
GCGCGGT65150.052.0296369
ACGGCCA26700.049.6562588
GCCATAC27050.049.14380611
GGGTCTA20150.048.512822
CCGAACT9800.048.50182767
CGGCCAT27550.048.2482459
TGGCGCG70350.048.1388815
CGCGGTG71200.047.70600510
GCCGGTT9900.046.2589343
GGTCTAC23700.046.001113
CGCGTAA306.6103024E-445.5726472
CCATACC29250.045.33068512
CATACCA29650.044.94965713
GGCCATA30100.044.27563510
CGTGCCT78600.043.33165420
TGAACGC30350.043.2029523