FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3301603
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG157530.4771318659451182No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN147010.44526855591056835No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG58760.17797415376712464No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG43720.13242052421202669No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA34690.10507017348845393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT14450.036.2231670
CGCGGCA46800.035.5310232
GCGGCAC48750.033.994133
TCGCCGG46450.033.84987348
CCCGTCG47550.033.3601444
CCGTCGC47400.033.34887745
TATCGGA1700.032.169968
CGGCACC51800.031.94933334
GTTCCGT4450.030.7535841
ACCCGCG55550.030.14127225
CCCCCGA55900.030.08580219
ATGGCCG14200.028.97421669
GCACCCC58600.028.6458336
CACCCCC58550.028.51635237
TCGTACG3600.028.48381812
GGCACCC61300.027.18919635
CGTCGCC58450.027.01595746
GTTATCG1900.026.9846696
CCCCGAC62500.026.98246620
AGTCCGC63850.026.498657
Produced by FastQC (version 0.11.5)