FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3399962
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG160160.47106408836334057No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN150110.44150493446691463No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG59350.17456077450277385No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG44060.12958968364940548No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA36460.10723649264315308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA49650.036.5096332
GCGGCAC50950.035.60790333
TCGCCGG49750.034.42059348
CGGCACC53400.033.87344434
CCGTCGC50800.033.75203745
CCCGTCG50800.033.7234644
TAGTCCG3650.031.8360186
ACCCGCG57800.031.66283225
CCCCCGA57800.031.53570419
GCACCCC60800.029.85913336
CACCCCC60950.029.8084737
GACGTAT4250.029.61510744
CCCCGAC62950.028.92273720
TGGCCGT15250.028.85273470
TATCGGA2400.028.4807518
GGCACCC63700.028.47412335
AGTCCGC66400.028.0518237
TTATCGG2450.027.8995117
CGTCGCC61750.027.79166846
CGTATCA4600.027.40943746