FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3375780
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG154370.45728690850707093No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN146390.4336479272938403No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG57810.1712493112702842No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG44100.13063647512574872No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA37760.11185563040245514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA48100.035.5113932
GCGGCAC50200.034.18521533
TCGCCGG48050.033.13210348
CGGCACC51800.032.82350534
TGGCCGT14600.032.78833470
CCCGTCG49400.032.3978844
CCGTCGC49400.032.35962345
CCCCCGA56850.030.00990719
ACCCGCG57750.029.83047925
GCACCCC58450.029.31675336
CACCCCC59950.028.3826337
GGCACCC61800.027.75940135
CCCCGAC62900.027.25184820
AGTCCGC65050.026.5915727
GTCGGTT3100.026.4645484
CGTCGCC60600.026.39906146
ATGGCCG14350.026.19860869
CAGTCCG63750.025.95416
GTCCGCC68150.025.5324638
TCCGCCC69900.025.4312089
Produced by FastQC (version 0.11.5)