FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3375780
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN145180.4300635704933379No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC134930.39970021743123074No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC106740.3161935908145673No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG94010.27848378745060404No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT52160.15451243860678124No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA38370.11366262019444394No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC36230.10732334453074548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG21150.060.9139335
GTCTACG21550.059.780614
TACGGCC22250.057.7505877
CTACGGC22550.057.2853856
CCGAACT8550.052.61282767
GCGCGGT64200.051.9525579
ACGGCCA25150.051.0914768
GCCATAC25050.050.74973711
CGGCCAT25400.050.5878529
TGGCGCG68150.049.39046515
GCCGGTT8700.049.0972373
GGGTCTA20200.047.3666762
CGCGGTG70900.047.1401810
CATACCA26950.047.0422313
CCATACC27250.046.52571512
GGCCATA28550.045.00702710
GAACGCG28100.044.3798524
TGAACGC28450.044.16509623
TACCACC28600.044.08928715
CGAACTT10550.043.82939568