FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5076844
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG235500.4638708615037216No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC142270.28023315272243937No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG122080.24046435147505024No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC115040.22659746882118104No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG97990.19301361239384154No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT79660.15690850457488945No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT66300.13059294317493308No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG63910.12588529409215646No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN62690.12348222635952572No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA59660.11751395158094281No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG56050.11040323476553544No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT56020.11034414293604451No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG55030.10839411256284416No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT54340.10703500048455301No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT25750.042.87045370
CGCGGCA74150.036.20691732
GCGGCAC77350.034.9909133
CGGCACC79300.033.8796934
TCGCCGG75700.032.76136848
ATGGCCG26550.032.7049969
CTTACCC17350.032.6886870
CCCGTCG77050.032.1197544
CCGTCGC77350.031.87350345
GCACCCC85500.031.56739636
CCCCCGA86250.031.20864119
ACCCGCG88650.030.40844325
CACCCCC90600.029.766637
GGCACCC91050.029.55789635
GTTCCGT4500.029.2235851
CCCCGAC93950.028.69435520
TACGGGT22150.028.3398196
GTACGGG22400.027.8690015
GTGCCGT7750.027.6855051
GTCGGTT3750.027.5918524