FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5076844
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG218840.43105519885976407No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC176240.3471448009826577No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA161360.31783525355516146No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC155320.3059380985509895No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT84150.16575258172203047No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN62630.12336404270054388No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG60160.11849881540579148No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC55260.10884714992227455No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC53040.10447435453994647No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCC46700.062.2365767
GTCTACG47300.061.8844534
TCTACGG47350.061.746295
CTACGGC48800.059.911626
GCCATAC52050.055.5094411
CGGCCAT52800.055.177519
ACGGCCA53600.054.2891048
CCATACC54800.052.91311312
GCGCGGT89700.052.773689
GGGTCTA41450.052.66492
CATACCA56700.051.01838713
GGCCATA57100.050.84264410
CCTGGTT83900.049.61482270
TGAACGC58250.049.5153823
TGGCGCG96150.049.41374615
TACCACC58900.049.34692815
GAACGCG58500.049.2534924
CTGAACG59850.049.04090522
CCGAACT14450.048.04335867
CGCGGTG99600.048.01415310