FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5085381
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG250110.49182155673291733No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC139970.27523994760667886No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG123160.24218441056825438No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC116970.23001226456778756No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG102890.2023250568639793No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT78210.15379378654224726No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT69590.1368432375076715No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG68960.13560439227660623No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN61820.12156414632453301No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA61050.120050002153231No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG56900.11188935499621365No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT54990.10813349088298399No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG53800.1057934498909718No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT53450.10510520254037997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT25600.038.70773370
CGCGGCA74950.036.82872432
GCGGCAC78650.035.19988333
TCGCCGG75000.034.57193448
CCCGTCG76400.033.95065744
CCGTCGC77250.033.5480245
CGGCACC82650.033.4233834
GCACCCC87000.031.93684436
ATGGCCG24650.031.86707969
CCCCCGA89750.031.42300819
AGGACCG7750.031.153870
CGTACGT2250.030.68369319
CACCCCC90650.030.6651837
GGCACCC91150.030.47071635
ACCCGCG92200.030.17679225
GTTCCGT5450.029.7820151
CCCCGAC98150.028.59811620
GTGCCGT8750.028.416931
TATCGGA1900.027.2334568
AGTCCGC105650.026.7737627