FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5221769
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG256580.49136604855557575No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC142150.27222575337974547No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG127730.24461059077871888No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC117930.22584300454501147No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG105260.20157919662857549No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT81220.15554115856139938No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG70970.13591179540879728No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT69980.1340158861872289No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN63550.12170205154613313No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA62250.11921247378043724No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG59020.11302683056259287No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT57660.11042234920771103No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT56380.10797107263841048No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG54530.10442821197184325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT24850.045.02945770
CGCGGCA80400.036.6184632
GCGGCAC82950.035.46192633
CGGCACC84550.034.64361234
CCCGTCG81050.034.57808344
TCGCCGG81550.034.45038248
CCGTCGC83550.033.56420545
ATGGCCG26550.033.38574669
GCACCCC93150.031.6925636
CCCCCGA94100.031.54961219
CACCCCC96600.030.4635237
ACCCGCG99100.030.0384525
GGCACCC98050.029.92030135
CCCCGAC103550.028.57612620
TATGGCC30500.027.13729968
GTATGGC30350.026.7300967
AGTCCGC112950.026.6370647
GTTCCGT5850.026.5781781
GATAGGC47750.026.48835840
CTTACCC17400.026.36102370