FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5221769
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG221760.4246836656313215No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC184270.3528880729882919No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA174140.3334885170140617No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC165740.3174020145280268No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT87340.16726132465836768No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN63420.12145309376956352No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG62080.11888691361107702No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC58590.11220335484009346No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC57760.11061385518968762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG46250.061.602085
GTCTACG46400.061.5510184
TACGGCC46650.061.0128067
CTACGGC48850.058.4673846
ACGGCCA51050.056.092018
CGGCCAT52850.053.789929
GCGCGGT94900.053.6221779
GCCATAC54600.052.5703711
GGGTCTA40450.052.528312
GGCCATA57100.050.2087510
CCATACC56950.050.09819812
CATACCA56950.049.85492713
CGCGGTG103150.049.53365310
TGGCGCG103950.049.40705515
CCTGGTT82800.049.06182570
GCCGGTT15350.048.9854243
GCGTCTA11500.048.5901642
CCGAACT15700.048.0482767
CGGTTCA15750.047.7422645
TGAACGC60100.047.30358523