FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3655043
Sequences flagged as poor quality0
Sequence length35-76
%GC61

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN223380.6111556006317846No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG177240.48491905567184845No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA163950.4485583343342336No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC128810.35241719454463327No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC116140.31775275968025546No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA67570.18486786612359965No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC59330.1623236717050935No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT55820.15272050150983177No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG48430.13250186112721518No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT47090.12883569358828337No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA37220.10183190731271835No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG36990.10120263975006588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG38300.061.3804474
TACGGCC38150.060.996767
TCTACGG39200.060.1445275
CTACGGC39950.058.845336
ACGGCCA41400.056.3724828
CGGCCAT41700.055.9676939
GCCATAC41450.055.894711
GGCCATA43050.054.2922710
CATACCA43250.053.72627313
GGGTCTA33050.053.461152
CCATACC43800.052.8965112
GCGTCTA7000.052.4242362
GCGCGGT62800.052.4180539
CCTGGTT63250.051.1082870
CCGAACT10000.050.53062467
CTGAACG45250.050.3504322
TACCACC46450.050.2444115
CGCGGTG65850.050.1972310
GCCGGTT9600.049.902743
CGGTTCA9700.049.382165
Produced by FastQC (version 0.11.5)