FastQCFastQC Report
Wed 8 May 2024
EGAF00005318976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3677892
Total Bases271.6 Mbp
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG316660.860982323570132No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC304710.8284908855398689No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCAC262050.7125005301950139No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN221700.6027909465530799No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG184480.5015916726211646No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT126970.34522492775753066No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG111170.3022655368890658No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG110790.30123233634919133No Hit
TGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGT98920.2689584142220598No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGT92380.25117648914106233No Hit
GTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCCT88900.24171454735484346No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG86660.2356241020671624No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT81450.22145837887572556No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA80810.21971825165067382No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT73060.19864639853481286No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA72890.1981841772406585No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC67260.18287649555778146No Hit
GCACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACC65680.17858055647093496No Hit
GGGGCTGGGGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCA64340.1749371650934829No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57340.15590452356947945No Hit
GGGGGCAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG56850.15457223866279923No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT55410.15065695240643281No Hit
TGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCG47800.12996575212105194No Hit
ACGTGCAGCTTGTCACAGTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTT44550.12112916855633608No Hit
GGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTG43580.11849178823086703No Hit
TGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA43450.1181383248882784No Hit
GTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGG41930.11400552272878052No Hit
CGCTTCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA41450.1127004273099917No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC41030.11155846881855151No Hit
GCCTGCACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACA41010.11150408984276863No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG40550.1102533733997627No Hit
AGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC39880.10843167771103664No Hit
GGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAG38520.10473390735780169No Hit
ACTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCG37280.10136241085926395No Hit
TGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCC36930.10041077878306377No Hit
GGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGT36780.10000293646469229No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)