FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3677892
Sequences flagged as poor quality0
Sequence length35-76
%GC61

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN220930.6006973559854395No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG177710.4831843893186641No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA168330.45768064967649946No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC134010.3643663272330998No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC119040.323663663859624No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA70740.1923384373440003No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC62440.16977116239411053No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT58880.16009170470476022No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG48960.13311973271645824No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT48930.13303816425278395No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG40000.10875795156573384No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA39890.10845886719892808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG39250.061.0944564
TACGGCC39650.060.478127
TCTACGG39650.060.3923345
CTACGGC40450.059.282016
ACGGCCA42350.056.7838
GCCATAC42400.056.3943911
CGGCCAT43200.055.508279
GGCCATA44100.054.68321610
GCGCGGT61500.054.421739
GGGTCTA32400.054.0707052
CATACCA44300.054.0524413
GCGTCTA8850.053.42832
CCATACC45550.052.64378712
CGCGGTG64850.051.55727410
CGTCTAC10050.050.767923
TACCACC47150.050.2081215
TGGCGCG66400.049.63660415
CCTGGTT64900.048.75044670
CTGAACG48400.048.2465822
ACTCTTC43350.048.1760985
Produced by FastQC (version 0.11.5)