FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3219196
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG38270.11888061491130084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA12100.037.7641532
TGGCCGT11900.037.66725570
TCGCCGG12950.034.75258648
CCGTCGC13400.033.5264445
CCCGTCG13650.033.14983444
GCGGCAC14250.032.0789233
GCGTATA553.279341E-431.3292584
CGGCACC14600.031.08484634
CGTTGGA6450.030.06692333
ATGGCCG10950.030.05978469
ACCCGCG15350.029.46384225
AGTCCGC16850.027.4061327
TCGTTGG7550.027.05180232
GATTTCG5450.026.63376428
CAGTCCG17100.025.997796
ATTTCGT5900.025.78436929
TTCGTTG7700.025.61367431
CGGGAAT5100.025.23059342
CGTCGCC18150.024.95790146
CCCCCGA18200.024.8198619
Produced by FastQC (version 0.11.5)